Quantitation of latent varicella-zoster virus DNA in human trigeminal ganglia by polymerase chain reaction.

نویسندگان

  • R Mahalingam
  • M Wellish
  • D Lederer
  • B Forghani
  • R Cohrs
  • D Gilden
چکیده

Competitive polymerase chain reaction was used to quantitate latent varicella-zoster virus (VZV) DNA in human trigeminal ganglia. Ganglionic DNA from five subjects was amplified with oligonucleotide primers specific for VZV gene 28. Two of the samples were also analyzed with primers specific for VZV gene 62. Our results indicated that there are 6 to 31 copies of the VZV genome in every 100,000 ganglionic cells.

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عنوان ژورنال:
  • Journal of virology

دوره 67 4  شماره 

صفحات  -

تاریخ انتشار 1993